Rev Bras Oftalmol.2021;80(1):08-11
Comparative study of inhibition of fibroblasts proliferation in vitro in the conjunctiva using mitomycin and ciclophosphamide
DOI: 10.5935/0034-7280.20210002
Abstract
Objective:
To evaluate the inhibition of fibroblast proliferation in vitro of conjunctiva obtained by excision of pterygium from patients using mitomycin (MMC) and cyclophosphamide (CF).
Methods:
Pterygiums were removed from 7 patients and subjected to cell culture. After cell cultivation, 3 fragments of equal dimensions of these tissues were collected from adjacent areas of each patient removed pterygium. They were randomly selected in such a way that one fragment of each patient was exposed to: the culture medium (group control), to MMC and to CF for an equal period of time at concentrations of 0,4 mg/dl and 10 mg/dl respectively. After this period, the fibroblast cell count of these groups were performed. Each group had seven fragments.
Results:
With the use of MMC we had a 95% rate of inhibition of fibroblast proliferation, while with CF 100%.
Conclusion:
Both drugs showed a high rate of inhibition of fibroblast proliferation, but CF showed greater inhibition than MMC.
